A Specific Micromethod for the Determination of Acyl Phosphates*
نویسندگان
چکیده
During the last few years it was observed that anhydrides of carboxylic and phosphoric acids appear as intermediates in metabolism. For one of these, acetyl phosphate, a method of determination had been worked out (I), based on a differential precipitation of calcium acetyl and inorganic phosphate with subsequent phosphate determination in the separate fractions. Such a procedure, founded on a more or less fortuitous behavior of the acetyl phosphate, lacked a desirable specificity for the characteristic anhydride group. In this communication, a method is described which utilizes the reaction of acyl phosphates with hydroxylamine. The acyl part of the acid anhydride is converted into hydroxamic acid.
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